About AnthOligo

AnthOligo is a web-based application developed to automatically generate oligo sequences to be used for the targeting and capturing the continuum of large and complex genomic regions. It automates the complex process of checking multiple applications so that the oligos selected successfully pass certain specific parameter criteria and then get shortlisted as a possible use case for RSE (region specific extraction) processes.

The tools listed below create the perfect foil for AnthOligo to produce optimal results !

  • Primer3
  • BLAT
  • UNAFold (Mfold) - Hairpin, Homodimer, Heterodimer analysis
  • MapDB
  • Apache ActiveMQ

Run Primer Design

Please type a name for this project:

Please type in your email address. Results will be emailed to you.


Optional Parameters:



Please upload your file in a tab-delimited file.

For help with file format or to download the example file, scroll up to find the Help page


Submit project for processing and creation of oligos.


For questions or comments please contact website admin:dgdbfx at email dot chop dot edu